New Step by Step Map For Orellanine
At last, one μCi of 86Rb was extra to each lifestyle and incubation was ongoing at 37 °C for yet another ten min. Reactions were terminated by washing the cells with ice-chilly 100 mM MgCl2 and cell lysates have been organized for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain were being subtracted from I